For its twentieth anniversary, ISBI will take place for the first time ever in Latin America. Located on the shores of the Caribbean Sea, Cartagena de Indias brings together the charm of colonial architecture, the excitement of vivid nightlife, fascinating cultural festivals, and lush landscapes. This year’s program will include five Keynotes Talks presented by world-renowned imaging scientists and clinicians: Drs. Matthew Rosen, Yakeel Quirov, Lena Maier-Hein, Samni Koyejo, and Vasilis Ntziachristos. Following recent ISBIs, we will also host the fourth edition of Clinical Day, a day-long special session designed to promote the exchange of emerging knowledge of biomedical imaging in the clinical fields. Additionally, we will hold the first-ever Industrial Day, in which we will hear about the latest products in our field by industry leaders, learn about the journey of medical imaging startups that have now become successful companies, and have a startup pitch competition. As in previous ISBIs, we also have an open call for Special Sessions, Challenges, and Tutorials, and we look forward to your submissions.